PROTOCOLS

Culturing cells in 3D is generating a lot of interest and change from the traditional way of culturing cells in 2D is imminent. To make this transition as smooth as possible detailed information on the use of 3D cell culture solutions is required. Because of this, we have put together a collection of protocols used successfully with our products, all available below.

GENERAL INSTRUCTIONS. HOW TO USE THE CELLEVATE 3D NANOMATRIX.

SHORT PRESENTATION. HOW COULD A CUSTOMIZED 3D IN-VITRO MODEL HELP YOUR ORGANIZATION?

GENERAL INSTRUCTIONS. PRETREATMENT & WASHING, PREINCUBATION & SEEDING, SEEDING DENSITIES.

GENERAL INSTRUCTIONS. PRETREATMENT & WASHING, PREINCUBATION & SEEDING, SEEDING DENSITIES. 

GENERAL INSTRUCTIONS. LEARN HOW YOU CAN LYSIS CELLS FROM THE SCAFFOLD.

GENERAL INSTRUCTIONS. LEARN HOW TO EXTRACT PROTEINS FOR WESTERN BLOT

GENERAL INSTRUCTIONS. USE ALAMARBLUE TO MEASURE CELL VIABILITY

GENERAL INSTRUCTIONS. PRETREATMENT & WASHING, PREINCUBATION & SEEDING, SEEDING DENSITIES. 

GENERAL INSTRUCTIONS. LAMININ COATING PROTOCOL FOR CULTURE WARE.

GENERAL INSTRUCTIONS. FOR OPTIMAL IMAGING OF CELLS, CONFOCAL MICROSCOPY IS RECOMMENDED.

GENERAL INSTRUCTIONS. LEARN HOW TO FIXATE CELLS FOR ELECTRON MICROSCOPY.

GENERAL INSTRUCTIONS. LEARN HOW TO EXTRACT PROTEINS FOR WESTERN BLOT

Watch this short introduction movie on how you best use your Cellevate 3D NanoMatrix plates.